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Tyrosine Hydroxylase 抗体 (pSer31)

Name: Tyrosine Hydroxylase 抗体 (pSer31)
Brand: antibodies-online
SKU: ABIN361502
Availability: OutOfStock

This anti-Tyrosine Hydroxylase antibody is a 兔多克隆 antibody detecting Tyrosine Hydroxylase in WB, IHC, ICC 和 ELISA. Suitable for 大鼠. This Primary Antibody has been cited in 5+ publications.

Western blots of PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 µM for 60 min) showing specific immunolabeling of the ~60k TH phosphorylated at Ser31. (Tyrosine Hydroxylase 抗体 (pSer31))

Western blot of PC-12 cell lysate incubated in the absence (-) and presence (+) of okadaic acid (OA, 1 µM for 60 min) showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase phosphorylated at Ser31. (Tyrosine Hydroxylase 抗体 (pSer31))

2 images

(5 references)

产品编号 ABIN361502

发货至: 中国

Quick Overview for Tyrosine Hydroxylase 抗体 (pSer31) (ABIN361502)

抗原

See all Tyrosine Hydroxylase (TH) 抗体

Tyrosine Hydroxylase (TH)

适用

大鼠

宿主

兔

克隆类型

多克隆

标记

This Tyrosine Hydroxylase antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA

Discover our top product TH Primary Antibody

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pSer31

特异性

Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser31.

交叉反应

人, 小鼠, 大鼠

预测反应

non-human primate

产品特性

Our Tyrosine Hydroxylase (Ser31) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Tyrosine Hydroxylase (Ser31) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC.

纯化方法

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

免疫原

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH).

亚型

IgG
anti-Tyrosine Hydroxylase (TH) (AA 101-165) antibody
TH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 82-310) antibody
TH 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (pSer40) antibody
TH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (pSer31) antibody
TH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) antibody
TH 适用: 人 WB, IF, ICC, IHC (p), IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 486-514) antibody
TH 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 34-301) antibody
TH 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (pSer19) antibody
TH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 193-222), (Middle Region) antibody
TH 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (C-Term) antibody
Verified TH 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

应用备注

Dilution Range: ICC: 1:50-1:300
Dilution Range: WB Brain: 1:1000
Dilution Range: WB: 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

储存条件

-20 °C

储存方法

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

有效期

12 months
Calipari, Ferris, Melchior, Bermejo, Salahpour, Roberts, Jones: "Methylphenidate and cocaine self-administration produce distinct dopamine terminal alterations." in: Addiction biology, Vol. 19, Issue 2, pp. 145-55, (2014) (PubMed).

Izumi, Ezumi, Takada-Takatori, Akaike, Kume: "Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 139, Issue 2, pp. 466-78, (2014) (PubMed).

Nakashima, Mori, Kaneko, Hayashi, Nagatsu, Ota: "Phosphorylation of the N-terminal portion of tyrosine hydroxylase triggers proteasomal digestion of the enzyme." in: Biochemical and biophysical research communications, Vol. 407, Issue 2, pp. 343-7, (2011) (PubMed).

Jedynak, Ali, Haycock, Hope: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." in: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, (2002) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).
抗原

Tyrosine Hydroxylase (TH)

别名

Tyrosine Hydroxylase

背景

Synonyms: Dystonia 14 antibody, DYT14 antibody, DYT5b antibody, EC 1.14.16.2 antibody, OTTHUMP00000011225 antibody, OTTHUMP00000011226 antibody, ple antibody, Protein Pale antibody, TH antibody, The antibody, TY3H_HUMAN antibody, TYH antibody, Tyrosine 3 hydroxylase antibody, Tyrosine 3 monooxygenase antibody, Tyrosine 3-hydroxylase antibody, Tyrosine 3-monooxygenase antibody, Tyrosine hydroxylase antibody
Description Logic: Our Tyrosine Hydroxylase (Ser31) rabbit polyclonal phosphospecific primary antibody is produced . It detects Tyrosine Hydroxylase (Ser31) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC.
Gene Name Alternatives: TH

分子量

60 kDa

基因ID

25085

UniProt

P04177

途径

Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour